› 3zbj

Electron Microscopy
8.5Å resolution

Fitting results in the I-layer of the subnanometer structure of the bacterial pKM101 type IV secretion system core complex digested with elastase

Released:
DOI: 10.2210/pdb3zbj/pdb
PDB entry 3zbj coloured by chain, front view.
PDB entry 3zbj coloured by chain, side view.
PDB entry 3zbj coloured by chain, top view.
Source organism: Escherichia coli BL21
Primary publication:
Structure of a bacterial type IV secretion core complex at subnanometre resolution.
Rivera-Calzada A, Fronzes R, Savva CG, Chandran V, Lian PW, Laeremans T, Pardon E, Steyaert J, Remaut H, Waksman G, Orlova EV
EMBO J 32 1195-204 (2013)
PMID: 23511972
Related structures: EMD-2233

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175134 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TraO protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 112 amino acids
Theoretical weight: 12.56 KDa
Source organism: Escherichia coli BL21
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q46704 (Residues: 24-135; Coverage: 41%)
Gene name: traO
Sequence domains: Conjugal transfer protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.5Å
Relevant EMDB volumes: EMD-2233
Expression system: Escherichia coli BL21

Quick links

Citations

26 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
25 more

2 mentions without citation

Recent advances in the structural and molecular biology of type IV secretion systems.
Trokter et al. (2014)
1 more