› 3wgk

X-ray diffraction
2.8Å resolution

STAPHYLOCOCCUS AUREUS FTSZ T7 mutant substituted for GAG, DeltaT7GAG-GDP

Released:
DOI: 10.2210/pdb3wgk/pdb
PDB entry 3wgk coloured by chain, front view.
PDB entry 3wgk coloured by chain, side view.
PDB entry 3wgk coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
Structural change in FtsZ Induced by intermolecular interactions between bound GTP and the T7 loop.
Matsui T, Han X, Yu J, Yao M, Tanaka I
J Biol Chem 289 3501-9 (2014)
PMID: 24347164

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140889 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division protein FtsZ Chains: A, B
Molecule details ›
Chains: A, B
Length: 390 amino acids
Theoretical weight: 40.97 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A029 (Residues: 1-390; Coverage: 100%)
Gene names: SAV1186, ftsZ
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 44.335Å b: 95.927Å c: 72.136Å
α: 90° β: 100.87° γ: 90°
R-values:
R R work R free
0.204 0.2 0.243
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Insights into the Structure, Function, and Dynamics of the Bacterial Cytokinetic FtsZ-Ring.
McQuillen et al. (2020)
7 more

3 mentions without citation

A Polymerization-Associated Structural Switch in FtsZ That Enables Treadmilling of Model Filaments.
Wagstaff et al. (2017)
2 more