3wfs

X-ray diffraction
3.31Å resolution

tRNA processing enzyme complex 3

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-130583 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CC-adding tRNA nucleotidyltransferase Chains: C, D
Molecule details ›
Chains: C, D
Length: 512 amino acids
Theoretical weight: 56.94 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
UniProt:
  • Canonical: O67911 (Residues: 16-448; Coverage: 85%)
Gene name: aq_2158
Sequence domains:
Structure domains: Beta Polymerase, domain 2
RNA (74-MER) Chains: A, B
Molecule details ›
Chains: A, B
Length: 74 nucleotides
Theoretical weight: 23.85 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2
Unit cell:
a: 177.21Å b: 148.11Å c: 92.25Å
α: 90° β: 98.54° γ: 90°
R-values:
R R work R free
0.231 0.229 0.276
Expression systems:
  • Escherichia coli
  • Not provided