› 3w7h

X-ray diffraction
1.67Å resolution

Structure of Trypanosoma cruzi dihydroorotate dehydrogenase in complex with MII-5-015

Released:
DOI: 10.2210/pdb3w7h/pdb
PDB entry 3w7h coloured by chain, front view.
PDB entry 3w7h coloured by chain, side view.
PDB entry 3w7h coloured by chain, top view.
Source organism: Trypanosoma cruzi strain CL Brener
Entry authors: Inaoka DK, Iida M, Tabuchi T, Lee N, Hashimoto S, Matsuoka S, Kuranaga T, Shiba T, Sakamoto K, Suzuki S, Balogun EO, Nara T, Aoki T, Inoue M, Honma T, Tanaka A, Harada S, Kita K

Function and Biology Details

Reaction catalysed: 
(S)-dihydroorotate + fumarate = orotate + succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175459 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroorotate dehydrogenase (fumarate) Chains: A, B
Molecule details ›
Chains: A, B
Length: 313 amino acids
Theoretical weight: 34.07 KDa
Source organism: Trypanosoma cruzi strain CL Brener
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4D3W2 (Residues: 2-314; Coverage: 100%)
Gene names: Tc00.1047053508375.50, pyr4, tcdhod2
Sequence domains: Dihydroorotate dehydrogenase
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
3 bound ligands:
Ligand W7H 2 x W7H
Ligand GOL 20 x GOL
Ligand NCO 1 x NCO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 67.908Å b: 71.72Å c: 129.111Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.143 0.176
Expression system: Escherichia coli

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