3w14 Summary

pdbe.org/3w14
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Insulin receptor ectodomain construct comprising domains L1,CR,L2, FNIII-1 and alphact peptide in complex with bovine insulin and FAB 83-14

There is a Quite Interesting Protein Structure article for this entry.

The structure was published by Menting, J.G., Whittaker, J., Margetts, M.B., et al., Weiss, M.A., Ward, C.W., and Lawrence, M.C., in 2013 in a paper entitled "How insulin engages its primary binding site on the insulin receptor" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 4.4 Å and deposited in 2012.

The experimental data on which the structure is based was also deposited.

This entry contains 10 copies of 5 unique biopolymers (complete list).

The most likely quaternary structure is a 20meric assembly.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Insulin A chain P01317 (85-105) (INS_BOVIN)search Bos taurussearch < 90% 21 100%
I Insulin A chain P01317 (85-105) (INS_BOVIN)search Bos taurussearch < 90% 21 100%
B Insulin B chain P01317 (25-54) (INS_BOVIN)search Bos taurussearch < 90% 30 56%
J Insulin B chain P01317 (25-54) (INS_BOVIN)search Bos taurussearch < 90% 30 56%
C MONOCLONAL ANTIBODY FAB 83-14 - HEAVY CHAIN Not available
Mus musculussearch Not available 207 93%
P MONOCLONAL ANTIBODY FAB 83-14 - HEAVY CHAIN Not available
Mus musculussearch Not available 207 93%
D MONOCLONAL ANTIBODY FAB 83-14 - LIGHT CHAIN Not available
Mus musculussearch Not available 214 100%
Q MONOCLONAL ANTIBODY FAB 83-14 - LIGHT CHAIN Not available
Mus musculussearch Not available 214 100%
E INSULIN RECEPTOR DOMAINS L1-CR-L2-(FNIII-1)-ALPHACT P06213 (28-746) (INSR_HUMAN)search Homo sapienssearch < 90% 609 95%
F INSULIN RECEPTOR DOMAINS L1-CR-L2-(FNIII-1)-ALPHACT P06213 (28-746) (INSR_HUMAN)search Homo sapienssearch < 90% 609 95%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P01317 (85 - 105) Insulin A chain Bos taurus
P06213 (28 - 746) INSULIN RECEPTOR DOMAINS L1-CR-L2-(FNIII-1)-ALPHACT Homo sapiens

Chain Sequence family (Pfam)
A, I Insulin/IGF/Relaxin familysearch
B, J Insulin/IGF/Relaxin familysearch
C, P
D, Q
E, F Furin-like cysteine rich regionsearch, Receptor L domainsearch

Chain ID Molecular function (GO) Cellular component (GO) Biological process (GO)
A, I (P01317) hormone activitysearch extracellular regionsearch
B, J (P01317) hormone activitysearch extracellular regionsearch
E, F (P06213) ATP bindingsearch transmembrane receptor protein tyrosine kinase activitysearch membranesearch protein phosphorylationsearch transmembrane receptor protein tyrosine kinase signaling pathwaysearch

Chain InterPro annotation
A, I Insulin-likesearch Insulin, conserved sitesearch
B, J Insulinsearch Insulin-likesearch
C, P Immunoglobulin V-set, subgroupsearch Immunoglobulin C1-setsearch Immunoglobulin-like domainsearch Immunoglobulin V-set domainsearch Immunoglobulin-like foldsearch
D, Q Immunoglobulin/major histocompatibility complex, conserved sitesearch Immunoglobulin V-set, subgroupsearch Immunoglobulin C1-setsearch Immunoglobulin-like domainsearch Immunoglobulin V-set domainsearch Immunoglobulin-like foldsearch
E, F EGF receptor, L domainsearch Fibronectin type IIIsearch Furin-like cysteine-rich domainsearch Furin-like repeatsearch Insulin-like growth factor binding protein, N-terminalsearch Immunoglobulin-like foldsearch