› 3w05

X-ray diffraction
1.58Å resolution

Crystal structure of Oryza sativa DWARF14 (D14) in complex with PMSF

Released:
DOI: 10.2210/pdb3w05/pdb
PDB entry 3w05 coloured by chain, front view.
PDB entry 3w05 coloured by chain, side view.
PDB entry 3w05 coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Structures of D14 and D14L in the strigolactone and karrikin signaling pathways.
Kagiyama M, Hirano Y, Mori T, Kim SY, Kyozuka J, Seto Y, Yamaguchi S, Hakoshima T
Genes Cells 18 147-60 (2013)
PMID: 23301669

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171258 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Strigolactone esterase D14 Chains: A, B
Molecule details ›
Chains: A, B
Length: 266 amino acids
Theoretical weight: 29.19 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q10QA5 (Residues: 55-318; Coverage: 83%)
Gene names: D14, D88, HTD2, LOC_Os03g10620, Os03g0203200
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand PMS 2 x PMS
Ligand EDO 7 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 48.3Å b: 88.518Å c: 118.884Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.194
Expression system: Escherichia coli

Quick links

Citations

45 review citations

Strigolactones, a novel carotenoid-derived plant hormone.
Al-Babili et al. (2015)
44 more

1 mention without citation

Target sites for chemical regulation of strigolactone signaling.
Nakamura et al. (2014)