› 3w01

X-ray diffraction
1.54Å resolution

Crystal structure of PcrB complexed with PEG from Staphylococcus aureus subsp. aureus Mu3

Released:
DOI: 10.2210/pdb3w01/pdb
PDB entry 3w01 coloured by chain, front view.
PDB entry 3w01 coloured by chain, side view.
PDB entry 3w01 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu3
Primary publication:
Insights into TIM-barrel prenyl transferase mechanisms: crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus.
Ren F, Feng X, Ko TP, Huang CH, Hu Y, Chan HC, Liu YL, Wang K, Chen CC, Pang X, He M, Li Y, Oldfield E, Guo RT
Chembiochem 14 195-9 (2013)
PMID: 23322418

Function and Biology Details

Reaction catalysed: 
All-trans-heptaprenyl diphosphate + sn-glycerol 1-phosphate = diphosphate + sn-3-O-(all-trans-heptaprenyl)glycerol 1-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108477 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heptaprenylglyceryl phosphate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 235 amino acids
Theoretical weight: 26.2 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu3
Expression system: Escherichia coli
UniProt:
  • Canonical: A7X435 (Residues: 1-230; Coverage: 100%)
Gene names: SAHV_1891, pcrB
Sequence domains: PcrB family
Structure domains: FMN-linked oxidoreductases

Ligands and Environments

1 bound ligand:
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 65.337Å b: 80.793Å c: 85.769Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.184 0.218
Expression system: Escherichia coli

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Citations

3 review citations

Biosynthesis of archaeal membrane ether lipids.
Jain et al. (2014)
2 more