› 3vof

X-ray diffraction
1.6Å resolution

Cellobiohydrolase mutant, CcCel6C D102A, in the closed form

Released:
DOI: 10.2210/pdb3vof/pdb
PDB entry 3vof coloured by chain, front view.
PDB entry 3vof coloured by chain, side view.
PDB entry 3vof coloured by chain, top view.
Source organism: Coprinopsis cinerea
Primary publication:
Comparison of the structural changes in two cellobiohydrolases, CcCel6A and CcCel6C, from Coprinopsis cinerea--a tweezer-like motion in the structure of CcCel6C.
Tamura M, Miyazaki T, Tanaka Y, Yoshida M, Nishikawa A, Tonozuka T
FEBS J 279 1871-82 (2012)
PMID: 22429290

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110643 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucanase Chain: A
Molecule details ›
Chain: A
Length: 395 amino acids
Theoretical weight: 43.59 KDa
Source organism: Coprinopsis cinerea
Expression system: Escherichia coli
UniProt:
  • Canonical: B7X9Z2 (Residues: 20-403; Coverage: 100%)
Gene name: CcCel6C
Sequence domains: Glycosyl hydrolases family 6
Structure domains: 1, 4-beta cellobiohydrolase

Ligands and Environments

1 bound ligand:
Ligand BGC 1 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P212121
Unit cell:
a: 49.906Å b: 79.281Å c: 94.996Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.184
Expression system: Escherichia coli

Quick links

Citations

10 citation in other articles

Crystal structure of a "loopless" GH19 chitinase in complex with chitin tetrasaccharide spanning the catalytic center.
Ohnuma et al. (2014)
9 more