› 3vhm

X-ray diffraction
2Å resolution

Crystal structure of NPC-biotin-avidin complex

Released:
DOI: 10.2210/pdb3vhm/pdb
PDB entry 3vhm coloured by chain, front view.
PDB entry 3vhm coloured by chain, side view.
PDB entry 3vhm coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Rational development of caged-biotin protein-labeling agents and some applications in live cells.
Terai T, Maki E, Sugiyama S, Takahashi Y, Matsumura H, Mori Y, Nagano T
Chem Biol 18 1261-72 (2011)
PMID: 22035795

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Avidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 123 amino acids
Theoretical weight: 13.72 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P02701 (Residues: 25-147; Coverage: 96%)
Gene name: AVD
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

3 bound ligands:
Ligand NPK 4 x NPK
Ligand NAG 4 x NAG
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 131.02Å b: 73.535Å c: 81.18Å
α: 90° β: 120.02° γ: 90°
R-values:
R R work R free
0.191 0.188 0.23

Quick links

Citations

1 review citation

Engineering Proteins at Interfaces: From Complementary Characterization to Material Surfaces with Designed Functions.
Morsbach et al. (2018)
8 other articles