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Raver1 in complex with metavinculin L954 deletion mutant

X-ray diffraction
2.54Å resolution
PDB entry 3vf0 coloured by chain, front view.
PDB entry 3vf0 coloured by chain, side view.
PDB entry 3vf0 coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Vinculin and Ribonucleoprotein PTB-binding 1
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Multimeric state: hetero dimer
Accessible surface area: 22059.35 Å2
Buried surface area: 5912.16 Å2
Dissociation area: 1,371.23 Å2
Dissociation energy (ΔGdiss): -0.97 kcal/mol
Dissociation entropy (TΔSdiss): 12.64 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148324

Macromolecules

Vinculin
Chain: A
Length: 283 amino acids
Theoretical weight: 31.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P18206 (Residues: 855-1134; Coverage: 25%)
Gene name: VCL
Pfam: Vinculin family
InterPro:
CATH: Alpha-catenin/vinculin-like
Vinculin in PDB entry 3vf0, assembly 1, front view.
Vinculin in PDB entry 3vf0, assembly 1, side view.
Vinculin in PDB entry 3vf0, assembly 1, top view.
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Ribonucleoprotein PTB-binding 1
Chain: B
Length: 285 amino acids
Theoretical weight: 31.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8IY67 (Residues: 39-321; Coverage: 47%)
Gene names: KIAA1978, RAVER1
Pfam: RNA recognition motif
InterPro:
CATH: Alpha-Beta Plaits
Ribonucleoprotein PTB-binding 1 in PDB entry 3vf0, assembly 1, front view.
Ribonucleoprotein PTB-binding 1 in PDB entry 3vf0, assembly 1, side view.
Ribonucleoprotein PTB-binding 1 in PDB entry 3vf0, assembly 1, top view.
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