› 3vek

X-ray diffraction
2.63Å resolution

Both Zn Fingers of GATA1 Bound to Palindromic DNA Recognition Site, P1 Crystal Form

Released:
DOI: 10.2210/pdb3vek/pdb
PDB entry 3vek coloured by chain, front view.
PDB entry 3vek coloured by chain, side view.
PDB entry 3vek coloured by chain, top view.
Source organisms:
Primary publication:
GATA1 directly mediates interactions with closely spaced pseudopalindromic but not distantly spaced double GATA sites on DNA.
Wilkinson-White L, Lester KL, Ripin N, Jacques DA, Mitchell Guss J, Matthews JM
Protein Sci 24 1649-59 (2015)
PMID: 26234528

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216170 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Erythroid transcription factor Chains: C, F
Molecule details ›
Chains: C, F
Length: 119 amino acids
Theoretical weight: 13.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P17679 (Residues: 200-318; Coverage: 29%)
Gene names: Gata1, Gf-1
Sequence domains: GATA zinc finger
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*TP*TP*GP*TP*CP*TP*TP*AP*TP*CP*AP*GP*AP*TP*GP*GP*AP*CP*TP*C)-3') Chains: A, D
Molecule details ›
Chains: A, D
Length: 20 nucleotides
Theoretical weight: 6.11 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*AP*AP*GP*AP*GP*TP*CP*CP*AP*TP*CP*TP*GP*AP*TP*AP*AP*GP*AP*C)-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 20 nucleotides
Theoretical weight: 6.15 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 14 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P1
Unit cell:
a: 35.195Å b: 62.415Å c: 66.046Å
α: 72.39° β: 74.57° γ: 73.63°
R-values:
R R work R free
0.237 0.235 0.275
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

GATA factor mutations in hematologic disease.
Crispino et al. (2017)
2 more

3 mentions without citation

Genome-Wide Organization of GATA1 and TAL1 Determined at High Resolution.
Han et al. (2016)
2 more