› 3vdh

X-ray diffraction
1.62Å resolution

Crystal structure of PbGH5A, a glycoside hydrolase family 5 enzyme from Prevotella bryantii B14

Released:
DOI: 10.2210/pdb3vdh/pdb
PDB entry 3vdh coloured by chain, front view.
PDB entry 3vdh coloured by chain, side view.
PDB entry 3vdh coloured by chain, top view.
Source organism: Prevotella bryantii
Primary publication:
Structure-Function Analysis of a Mixed-linkage β-Glucanase/Xyloglucanase from the Key Ruminal Bacteroidetes Prevotella bryantii B(1)4.
McGregor N, Morar M, Fenger TH, Stogios P, Lenfant N, Yin V, Xu X, Evdokimova E, Cui H, Henrissat B, Savchenko A, Brumer H
J Biol Chem 291 1175-97 (2016)
PMID: 26507654

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126540 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH26 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 353 amino acids
Theoretical weight: 40.04 KDa
Source organism: Prevotella bryantii
Expression system: Escherichia coli
UniProt:
  • Canonical: O06842 (Residues: 573-924; Coverage: 39%)
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand CL 3 x CL
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 57.908Å b: 85.254Å c: 74.556Å
α: 90° β: 109.15° γ: 90°
R-values:
R R work R free
0.152 0.151 0.199
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Recent structural insights into the enzymology of the ubiquitous plant cell wall glycan xyloglucan.
Attia et al. (2016)
2 more

7 mentions without citation

Metadata Analysis Approaches for Understanding and Improving the Functional Involvement of Rumen Microbial Consortium in Digestion and Metabolism of Plant Biomass.
Kameshwar et al. (2019)
6 more