› 3vcn

X-ray diffraction
1.45Å resolution

Crystal structure of mannonate dehydratase (target EFI-502209) from Caulobacter crescentus CB15

Released:
DOI: 10.2210/pdb3vcn/pdb
PDB entry 3vcn coloured by chain, front view.
PDB entry 3vcn coloured by chain, side view.
PDB entry 3vcn coloured by chain, top view.
Source organism: Caulobacter vibrioides
Entry authors: Patskovsky Y, Toro R, Bhosle R, Hillerich B, Seidel RD, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Zencheck WD, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-189509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-mannonate dehydratase CC0532 Chains: A, C
Molecule details ›
Chains: A, C
Length: 425 amino acids
Theoretical weight: 47.34 KDa
Source organism: Caulobacter vibrioides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AAR4 (Residues: 1-403; Coverage: 100%)
Gene name: CC_0532
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 4 x MG
Ligand GOL 3 x GOL
Ligand CO3 2 x CO3
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I4
Unit cell:
a: 116.302Å b: 116.302Å c: 118.078Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.132 0.13 0.188
Expression system: Escherichia coli BL21(DE3)

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