› 3v7a

X-ray diffraction
3.3Å resolution

Structural basis for broad detection of genogroup II noroviruses by a monoclonal antibody that binds to a site occluded in the viral particle

Released:
DOI: 10.2210/pdb3v7a/pdb
PDB entry 3v7a coloured by chain, front view.
PDB entry 3v7a coloured by chain, side view.
PDB entry 3v7a coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for broad detection of genogroup II noroviruses by a monoclonal antibody that binds to a site occluded in the viral particle.
Hansman GS, Taylor DW, McLellan JS, Smith TJ, Georgiev I, Tame JR, Park SY, Yamazaki M, Gondaira F, Miki M, Katayama K, Murata K, Kwong PD
J Virol 86 3635-46 (2012)
PMID: 22278249

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212304 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
5B18 heavy chain Chains: E, F
Molecule details ›
Chains: E, F
Length: 223 amino acids
Theoretical weight: 23.71 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
5B18 kappa chain Chains: G, H
Molecule details ›
Chains: G, H
Length: 215 amino acids
Theoretical weight: 23.77 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Capsid protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 34.51 KDa
Source organism: Norovirus isolates
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5F4T5 (Residues: 224-538; Coverage: 58%)
Sequence domains: Calicivirus coat protein C-terminal
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P4322
Unit cell:
a: 145.48Å b: 145.48Å c: 216.334Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.227 0.283
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Single-Domain Antibodies As Therapeutics against Human Viral Diseases.
Wu et al. (2017)
8 more

6 mentions without citation

Rapid emergence and predominance of a broadly recognizing and fast-evolving norovirus GII.17 variant in late 2014.
Chan et al. (2015)
5 more