› 3v4q

X-ray diffraction
3.06Å resolution

Structure of R335W mutant of human Lamin

Released:
DOI: 10.2210/pdb3v4q/pdb
PDB entry 3v4q coloured by chain, front view.
PDB entry 3v4q coloured by chain, side view.
PDB entry 3v4q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the lamin A/C R335W and E347K mutants: implications for dilated cardiolaminopathies.
Bollati M, Barbiroli A, Favalli V, Arbustini E, Charron P, Bolognesi M
Biochem Biophys Res Commun 418 217-21 (2012)
PMID: 22266370

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-135892 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lamin-A/C Chain: A
Molecule details ›
Chain: A
Length: 74 amino acids
Theoretical weight: 8.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02545 (Residues: 313-386; Coverage: 11%)
Gene names: LMN1, LMNA
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P6522
Unit cell:
a: 90.499Å b: 90.499Å c: 75.425Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.276 0.275 0.282
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Cellular Mechanotransduction: From Tension to Function.
Martino et al. (2018)
7 more

4 mentions without citation

Recent insight into intermediate filament structure.
Eldirany et al. (2021)
3 more