› 3v4f

X-ray diffraction
1.39Å resolution

H-Ras PEG 400/CaCl2, ordered off

Released:
DOI: 10.2210/pdb3v4f/pdb
PDB entry 3v4f coloured by chain, front view.
PDB entry 3v4f coloured by chain, side view.
PDB entry 3v4f coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Shift in the equilibrium between on and off states of the allosteric switch in Ras-GppNHp affected by small molecules and bulk solvent composition.
Holzapfel G, Buhrman G, Mattos C
Biochemistry 51 6114-26 (2012)
PMID: 22845804

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.88 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P20171 (Residues: 1-166; Coverage: 88%)
Gene names: Hras, Hras1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand CA 2 x CA
Ligand MG 2 x MG
Ligand DTU 1 x DTU
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS
Spacegroup: R32
Unit cell:
a: 88.521Å b: 88.521Å c: 134.06Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.183 0.198
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Drugging Ras GTPase: a comprehensive mechanistic and signaling structural view.
Lu et al. (2016)
4 more

4 mentions without citation

A structural model of a Ras-Raf signalosome.
Mysore et al. (2021)
3 more