› 3v3g

X-ray diffraction
1.56Å resolution

Kinetic and structural studies of thermostabilized mutants of HCA II.

Released:
DOI: 10.2210/pdb3v3g/pdb
PDB entry 3v3g coloured by chain, front view.
PDB entry 3v3g coloured by chain, side view.
PDB entry 3v3g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Kinetic and structural characterization of thermostabilized mutants of human carbonic anhydrase II.
Fisher Z, Boone CD, Biswas SM, Venkatakrishnan B, Aggarwal M, Tu C, Agbandje-McKenna M, Silverman D, McKenna R
Protein Eng Des Sel 25 347-55 (2012)
PMID: 22691706

Function and Biology Details

Reactions catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Urea = cyanamide + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133681 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chain: B
Molecule details ›
Chain: B
Length: 260 amino acids
Theoretical weight: 29.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00918 (Residues: 1-260; Coverage: 100%)
Gene name: CA2
Sequence domains: Eukaryotic-type carbonic anhydrase
Structure domains: Alpha carbonic anhydrase

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 42.05Å b: 72.057Å c: 74.701Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.2
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structural annotation of human carbonic anhydrases.
Aggarwal et al. (2013)
4 more

1 mention without citation

Kinetic and structural characterization of thermostabilized mutants of human carbonic anhydrase II.
Fisher et al. (2012)