› 3uto

X-ray diffraction
2.4Å resolution

Twitchin kinase region from C.elegans (Fn31-NL-kin-CRD-Ig26)

Released:
DOI: 10.2210/pdb3uto/pdb
PDB entry 3uto coloured by chain, front view.
PDB entry 3uto coloured by chain, side view.
PDB entry 3uto coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Identification of an N-terminal inhibitory extension as the primary mechanosensory regulator of twitchin kinase.
von Castelmur E, Strümpfer J, Franke B, Bogomolovas J, Barbieri S, Qadota H, Konarev PV, Svergun DI, Labeit S, Benian GM, Schulten K, Mayans O
Proc Natl Acad Sci U S A 109 13608-13 (2012)
PMID: 22869697

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173159 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Twitchin Chains: A, B
Molecule details ›
Chains: A, B
Length: 573 amino acids
Theoretical weight: 65.25 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q23551 (Residues: 6108-6111, 6112-6675, 6107-6111; Coverage: 8%)
Gene names: ZK617.1, unc-22
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GOL 1 x GOL
Ligand PEG 1 x PEG
Ligand FLC 1 x FLC
Ligand P33 1 x P33
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21212
Unit cell:
a: 121.7Å b: 158.21Å c: 60.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.213
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Molecular stretching modulates mechanosensing pathways.
Hu et al. (2017)
3 more

5 mentions without citation

Titin kinase is an inactive pseudokinase scaffold that supports MuRF1 recruitment to the sarcomeric M-line.
Bogomolovas et al. (2014)
4 more