3uon Summary


Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist

The structure was published by Haga, K., Kruse, A.C., Asada, H., et al., Kobilka, B.K., Haga, T., and Kobayashi, T., in 2012 in a paper entitled "Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.0 Å and deposited in 2011.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of Human M2 muscarinic acetylcholine, receptor T4 lysozyme fusion protein. This molecule has the UniProt identifier P08172 (ACM2_HUMAN)search. The sample contained 467 residues which is 98% of the natural sequence. Out of 467 residues 434 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Human M2 muscarinic acetylcholine, receptor T4 lysozyme fusion protein P00720 (2-161) (ENLYS_BPT4)search ,
P08172 (1-217) (ACM2_HUMAN)search ,
P08172 (377-466) (ACM2_HUMAN)search
Enterobacteria phage T4search ,
Homo sapienssearch ,
Homo sapienssearch
93% ,
< 90% ,
< 90%
467 93%

Chain Sequence family (Pfam)
A (P00720) PF00959: Phage lysozymesearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A (P08172P00720) cell wall macromolecule catabolic processsearch regulation of heart contractionsearch peptidoglycan catabolic processsearch metabolic processsearch defense response to bacteriumsearch cytolysissearch viral release from host cellsearch G-protein coupled receptor signaling pathwaysearch G-protein coupled receptor activitysearch lysozyme activitysearch G-protein coupled acetylcholine receptor activitysearch hydrolase activitysearch hydrolase activity, acting on glycosyl bondssearch catalytic activitysearch integral component of membranesearch host cell cytoplasmsearch

Chain InterPro annotation
A G protein-coupled receptor, rhodopsin-likesearch Muscarinic acetylcholine receptor M2search Bacteriophage T4, Gp5, tail-associated lysozymesearch Glycoside hydrolase, family 24search GPCR, rhodopsin-like, 7TMsearch Lysozyme-like domainsearch Lysozyme domainsearch