› 3un2

X-ray diffraction
1.8Å resolution

Phosphopentomutase T85Q variant enzyme

Released:
DOI: 10.2210/pdb3un2/pdb
PDB entry 3un2 coloured by chain, front view.
PDB entry 3un2 coloured by chain, side view.
PDB entry 3un2 coloured by chain, top view.
Source organism: Bacillus cereus
Primary publication:
Molecular differences between a mutase and a phosphatase: investigations of the activation step in Bacillus cereus phosphopentomutase.
Iverson TM, Panosian TD, Birmingham WR, Nannemann DP, Bachmann BO
Biochemistry 51 1964-75 (2012)
PMID: 22329805

Function and Biology Details

Reaction catalysed: 
Alpha-D-ribose 1-phosphate = D-ribose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182450 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphopentomutase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 399 amino acids
Theoretical weight: 44.54 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q818Z9 (Residues: 2-394; Coverage: 100%)
Gene names: BC_4087, deoB
Sequence domains: Metalloenzyme superfamily
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MN 9 x MN
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 91.897Å b: 76.72Å c: 107.19Å
α: 90° β: 108.68° γ: 90°
R-values:
R R work R free
0.169 0.167 0.194
Expression system: Escherichia coli BL21

Quick links

Citations

5 citation in other articles

Bioretrosynthetic construction of a didanosine biosynthetic pathway.
Birmingham et al. (2014)
4 more

1 mention without citation

Molecular differences between a mutase and a phosphatase: investigations of the activation step in Bacillus cereus phosphopentomutase.
Iverson et al. (2012)