› 3um7

X-ray diffraction
3.31Å resolution

Crystal structure of the human two pore domain K+ ion channel TRAAK (K2P4.1)

Released:
DOI: 10.2210/pdb3um7/pdb
PDB entry 3um7 coloured by chain, front view.
PDB entry 3um7 coloured by chain, side view.
PDB entry 3um7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human K2P TRAAK, a lipid- and mechano-sensitive K+ ion channel.
Brohawn SG, del Mármol J, MacKinnon R
Science 335 436-41 (2012)
PMID: 22282805

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192705 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium channel subfamily K member 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 33.59 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9NYG8 (Residues: 1-274; Coverage: 70%)
Gene names: KCNK4, TRAAK
Sequence domains: Ion channel
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand K 5 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 87.938Å b: 130.852Å c: 132.779Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.317 0.317 0.323
Expression system: Komagataella pastoris

Quick links

Citations

70 review citations

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production.
Ahmad et al. (2014)
69 more

27 mentions without citation

The recombinant expression systems for structure determination of eukaryotic membrane proteins.
He et al. (2014)
26 more