› 3uko

X-ray diffraction
1.4Å resolution

Crystal Structure of S-Nitrosoglutathione Reductase from Arabidopsis thaliana, complex with NADH

Released:
DOI: 10.2210/pdb3uko/pdb
PDB entry 3uko coloured by chain, front view.
PDB entry 3uko coloured by chain, side view.
PDB entry 3uko coloured by chain, top view.
Source organism: Arabidopsis thaliana
Entry authors: Weichsel A, Crotty J, Montfort WR

Function and Biology Details

Reactions catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
S-(hydroxymethyl)glutathione + NAD(P)(+) = S-formylglutathione + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-188313 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase class-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 378 amino acids
Theoretical weight: 40.62 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96533 (Residues: 2-379; Coverage: 100%)
Gene names: ADH2, ADHIII, At5g43940, FDH1, MRH10.4
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
Ligand ZN 4 x ZN
Ligand SO4 12 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P3121
Unit cell:
a: 92.244Å b: 92.244Å c: 172.872Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.18 0.234
Expression system: Escherichia coli

Quick links