› 3uk6

X-ray diffraction
2.95Å resolution

Crystal Structure of the Tip48 (Tip49b) hexamer

Released:
DOI: 10.2210/pdb3uk6/pdb
PDB entry 3uk6 coloured by chain, front view.
PDB entry 3uk6 coloured by chain, side view.
PDB entry 3uk6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Large-scale conformational flexibility determines the properties of AAA+ TIP49 ATPases.
Petukhov M, Dagkessamanskaja A, Bommer M, Barrett T, Tsaneva I, Yakimov A, Quéval R, Shvetsov A, Khodorkovskiy M, Käs E, Grigoriev M
Structure 20 1321-31 (2012)
PMID: 22748767

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195085 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RuvB-like 2 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 368 amino acids
Theoretical weight: 40.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y230 (Residues: 1-132, 239-463; Coverage: 77%)
Gene names: CGI-46, INO80J, RUVBL2, TIP48, TIP49B
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 12 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 113.07Å b: 186.28Å c: 129.86Å
α: 90° β: 108.95° γ: 90°
R-values:
R R work R free
0.242 0.241 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Chaperone-like activity of the AAA+ proteins Rvb1 and Rvb2 in the assembly of various complexes.
Nano et al. (2013)
1 more

6 mentions without citation

AAA ATPases as therapeutic targets: Structure, functions, and small-molecule inhibitors.
Zhang et al. (2021)
5 more