3ugm

X-ray diffraction
3Å resolution

Structure of TAL effector PthXo1 bound to its DNA target

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-109724 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
TAL effector protein PthXo1 Chain: A
Molecule details ›
Chain: A
Length: 1047 amino acids
Theoretical weight: 108.9 KDa
Source organism: Xanthomonas oryzae pv. oryzae PXO99A
Expression system: Escherichia coli
UniProt:
  • Canonical: B2SU53 (Residues: 127-1149; Coverage: 75%)
Gene names: PXO_00227, pthXo1, tal2b
Sequence domains: TAL effector repeat
DNA-1 Chain: B
Molecule details ›
Chain: B
Length: 38 nucleotides
Theoretical weight: 11.53 KDa
Source organism: Xanthomonas oryzae pv. oryzae PXO99A
Expression system: Not provided
DNA-2 Chain: C
Molecule details ›
Chain: C
Length: 38 nucleotides
Theoretical weight: 11.86 KDa
Source organism: Xanthomonas oryzae pv. oryzae PXO99A
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 95.578Å b: 248.481Å c: 54.646Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.266 0.266 0.294
Expression systems:
  • Escherichia coli
  • Not provided