› 3uac

X-ray diffraction
1.3Å resolution

Multicopper Oxidase CueO mutant C500SE506Q (data4)

Released:
DOI: 10.2210/pdb3uac/pdb
PDB entry 3uac coloured by chain, front view.
PDB entry 3uac coloured by chain, side view.
PDB entry 3uac coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
An O-centered structure of the trinuclear copper center in the Cys500Ser/Glu506Gln mutant of CueO and structural changes in low to high X-ray dose conditions.
Komori H, Sugiyama R, Kataoka K, Higuchi Y, Sakurai T
Angew Chem Int Ed Engl 51 1861-4 (2012)
PMID: 22250058

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multicopper oxidase CueO Chain: A
Molecule details ›
Chain: A
Length: 489 amino acids
Theoretical weight: 53.52 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P36649 (Residues: 29-516; Coverage: 100%)
Gene names: JW0119, b0123, cueO, yacK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

3 bound ligands:
Ligand CU 3 x CU
Ligand O 2 x O
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P21
Unit cell:
a: 50.475Å b: 91.181Å c: 53.312Å
α: 90° β: 102.91° γ: 90°
R-values:
R R work R free
0.144 0.144 not available
Expression system: Escherichia coli

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Citations

1 review citation

Radiation chemists look at damage in redox proteins induced by X-rays.
Wherland et al. (2018)
9 other articles