› 3u85

X-ray diffraction
3Å resolution

Crystal structure of human menin in complex with MLL1

Released:
DOI: 10.2210/pdb3u85/pdb
PDB entry 3u85 coloured by chain, front view.
PDB entry 3u85 coloured by chain, side view.
PDB entry 3u85 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The same pocket in menin binds both MLL and JUND but has opposite effects on transcription.
Huang J, Gurung B, Wan B, Matkar S, Veniaminova NA, Wan K, Merchant JL, Hua X, Lei M
Nature 482 542-6 (2012)
PMID: 22327296

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a [histone H3]-L-lysine(4) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(4)
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-126203 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Menin Chain: A
Molecule details ›
Chain: A
Length: 550 amino acids
Theoretical weight: 61.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00255 (Residues: 2-610; Coverage: 90%)
Gene names: MEN1, SCG2
Sequence domains: Menin
MLL cleavage product N320 Chain: B
Molecule details ›
Chain: B
Length: 21 amino acids
Theoretical weight: 2.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03164 (Residues: 6-25; Coverage: 1%)
Gene names: ALL1, CXXC7, HRX, HTRX, KMT2A, MLL, MLL1, TRX1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P41212
Unit cell:
a: 141.205Å b: 141.205Å c: 93.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.22 0.258
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

Quick links

Citations

55 review citations

Epigenetic modifications of histones in cancer.
Zhao et al. (2019)
54 more

7 mentions without citation

Small-molecule inhibitors of protein-protein interactions: progressing toward the reality.
Arkin et al. (2014)
6 more