› 3u5t

X-ray diffraction
2.4Å resolution

The crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase from Sinorhizobium meliloti

Released:
DOI: 10.2210/pdb3u5t/pdb
PDB entry 3u5t coloured by chain, front view.
PDB entry 3u5t coloured by chain, side view.
PDB entry 3u5t coloured by chain, top view.
Source organism: Sinorhizobium meliloti
Entry authors: Zhang Z, Chamala S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, LaFleur J, Seidel R, Villigas G, Zencheck W, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187943 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 267 amino acids
Theoretical weight: 28.02 KDa
Source organism: Sinorhizobium meliloti
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92W70 (Residues: 1-244; Coverage: 100%)
Gene name: SM_b20493
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 93.173Å b: 95.397Å c: 112.694Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.244 0.285
Expression system: Escherichia coli BL21(DE3)

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