3u43 Summary


Crystal structure of the colicin E2 DNase-Im2 complex

The structure was published by Wojdyla, J.A., Fleishman, S.J., Baker, D., and Kleanthous, C., in 2012 in a paper entitled "Structure of the Ultra-High-Affinity Colicin E2 DNase-Im2 Complex." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.72 Å and deposited in 2011.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Colicin-E2 immunity protein and Colicin-E2.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Colicin-E2 immunity protein P04482 (1-86) (IMM2_ECOLX)search Escherichia colisearch 100% 94 97%
B Colicin-E2 P04419 (449-581) (CEA2_ECOLX)search Escherichia colisearch < 90% 134 98%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P04482 (1 - 86) Colicin-E2 immunity protein Escherichia coli
P04419 (449 - 581) Colicin-E2 Escherichia coli

Chain Structural classification (CATH) Sequence family (Pfam)
A (P04482) PF01320: Colicin immunity protein / pyocin immunity proteinsearch
B Colicin e7 immunity protein. Chain Bsearch DNase/tRNase domain of colicin-like bacteriocinsearch

Chain ID Molecular function (GO) Biological process (GO)
A (P04482) toxic substance bindingsearch bacteriocin immunitysearch
B (P04419) endonuclease activitysearch receptor bindingsearch cytolysissearch response to bacteriumsearch pathogenesissearch

Chain InterPro annotation
A Colicin immunity protein/pyocin immunity proteinsearch Colicin/pyocin immunity protein domainsearch
B Pyocin S killer proteinsearch HNH nucleasesearch Colicin/pyocin, DNase domainsearch