3u25

X-ray diffraction
1.18Å resolution

Crystal structure of P. aeruginoas azurin containing a Tyr-His hydrogen bonded pair

Released:
Source organism: Pseudomonas aeruginosa
Primary publication:
Redox properties of tyrosine and related molecules.
FEBS Lett 586 596-602 (2012)
PMID: 22210190

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 13.86 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00282 (Residues: 22-148; Coverage: 99%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 48.026Å b: 56.33Å c: 82.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.195 0.217
Expression system: Escherichia coli BL21(DE3)