› 3tqh

X-ray diffraction
2.44Å resolution

Structure of the quinone oxidoreductase from Coxiella burnetii

Released:
DOI: 10.2210/pdb3tqh/pdb
PDB entry 3tqh coloured by chain, front view.
PDB entry 3tqh coloured by chain, side view.
PDB entry 3tqh coloured by chain, top view.
Source organism: Coxiella burnetii
Primary publication:
Structural genomics for drug design against the pathogen Coxiella burnetii.
Franklin MC, Cheung J, Rudolph MJ, Burshteyn F, Cassidy M, Gary E, Hillerich B, Yao ZK, Carlier PR, Totrov M, Love JD
Proteins 83 2124-36 (2015)
PMID: 26033498

Function and Biology Details

Reaction catalysed: 
NADPH + 2 quinone = NADP(+) + 2 semiquinone
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182920 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl reductase (ER) domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 35.06 KDa
Source organism: Coxiella burnetii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q83CT0 (Residues: 1-318; Coverage: 100%)
Gene name: CBU_1023
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P43212
Unit cell:
a: 52.911Å b: 52.911Å c: 221.122Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.254
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Laccases of prokaryotic origin: enzymes at the interface of protein science and protein technology.
Martins et al. (2015)
3 more

4 mentions without citation

Structural basis for the enzymatic formation of the key strawberry flavor compound 4-hydroxy-2,5-dimethyl-3(2H)-furanone.
Schiefner et al. (2013)
3 more