› 3tot

X-ray diffraction
1.76Å resolution

Crystal structure of GLUTATHIONE TRANSFERASE (TARGET EFI-501058) from Ralstonia solanacearum GMI1000

Released:
DOI: 10.2210/pdb3tot/pdb
PDB entry 3tot coloured by chain, front view.
PDB entry 3tot coloured by chain, side view.
PDB entry 3tot coloured by chain, top view.
Source organism: Ralstonia solanacearum
Entry authors: Patskovsky Y, Toro R, Bhosle R, Zencheck WD, Hillerich B, Seidel RD, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Imker HJ, Armstrong RN, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186918 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable glutathione s-transferase protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 226 amino acids
Theoretical weight: 26.05 KDa
Source organism: Ralstonia solanacearum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8XVV6 (Residues: 1-202; Coverage: 100%)
Gene names: RSc2721, gstK
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ACT 10 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P41212
Unit cell:
a: 76.324Å b: 76.324Å c: 151.624Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.204
Expression system: Escherichia coli BL21(DE3)

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