› 3thf

X-ray diffraction
2.7Å resolution

Crystal structure of the SD2 domain from Drosophila Shroom

Released:
DOI: 10.2210/pdb3thf/pdb
PDB entry 3thf coloured by chain, front view.
PDB entry 3thf coloured by chain, side view.
PDB entry 3thf coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structure of Shroom domain 2 reveals a three-segmented coiled-coil required for dimerization, Rock binding, and apical constriction.
Mohan S, Rizaldy R, Das D, Bauer RJ, Heroux A, Trakselis MA, Hildebrand JD, VanDemark AP
Mol Biol Cell 23 2131-42 (2012)
PMID: 22493320

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-106778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein Shroom Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 21.37 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A1Z9P3 (Residues: 1393-1576; Coverage: 12%)
Gene names: CG34379, Shrm, Shroom
Sequence domains: Apx/Shroom domain ASD2
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 72.797Å b: 85.632Å c: 92.977Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.228 0.284
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Dynamic contacts: rearranging adherens junctions to drive epithelial remodelling.
Takeichi M. (2014)
6 more

24 mentions without citation

Rare Earth Starting Materials and Methodologies for Synthetic Chemistry.
Ortu F. (2022)
23 more