› 3th1

X-ray diffraction
3.4Å resolution

Crystal structure of chlorocatechol 1,2-dioxygenase from Pseudomonas putida

Released:
DOI: 10.2210/pdb3th1/pdb
PDB entry 3th1 coloured by chain, front view.
PDB entry 3th1 coloured by chain, side view.
PDB entry 3th1 coloured by chain, top view.
Source organism: Pseudomonas putida
Entry authors: Rustiguel JK, Nonato MC

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145842 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chlorocatechol 1,2-dioxygenase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 260 amino acids
Theoretical weight: 29.02 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11451 (Residues: 1-260; Coverage: 100%)
Gene name: clcA
Sequence domains:
Structure domains: Aromatic compound dioxygenase

Ligands and Environments

6 bound ligands:
Ligand FE 3 x FE
Ligand DTD 2 x DTD
Ligand 3PH 2 x 3PH
Ligand MG 2 x MG
Ligand ACT 1 x ACT
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P6122
Unit cell:
a: 97.57Å b: 97.57Å c: 423.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.226 0.279
Expression system: Escherichia coli BL21(DE3)

Quick links