› 3tg2

X-ray diffraction
1.1Å resolution

Crystal structure of the ISC domain of VibB in complex with isochorismate

Released:
DOI: 10.2210/pdb3tg2/pdb
PDB entry 3tg2 coloured by chain, front view.
PDB entry 3tg2 coloured by chain, side view.
PDB entry 3tg2 coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Structural insight into the ISC domain of VibB from Vibrio cholerae at atomic resolution: a snapshot just before the enzymatic reaction.
Liu S, Zhang C, Li N, Niu B, Liu M, Liu X, Wei T, Zhu D, Huang Y, Xu S, Gu L
Acta Crystallogr D Biol Crystallogr 68 1329-38 (2012)
PMID: 22993087

Function and Biology Details

Reaction catalysed: 
Isochorismate + H(2)O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vibriobactin-specific isochorismatase Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 24.84 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C6D3 (Residues: 1-215; Coverage: 73%)
Gene names: VC_0771, vibB
Sequence domains: Isochorismatase family
Structure domains: Isochorismatase-like

Ligands and Environments

2 bound ligands:
Ligand ISC 1 x ISC
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3221
Unit cell:
a: 55.584Å b: 55.584Å c: 119.792Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.128 0.127 0.144
Expression system: Escherichia coli

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Citations

3 citation in other articles

Unconventionally secreted effectors of two filamentous pathogens target plant salicylate biosynthesis.
Liu et al. (2014)
2 more