› 3tfc

X-ray diffraction
1.95Å resolution

1.95 Angstrom crystal structure of a bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase (aroA) from Listeria monocytogenes EGD-e in complex with phosphoenolpyruvate

Released:
DOI: 10.2210/pdb3tfc/pdb
PDB entry 3tfc coloured by chain, front view.
PDB entry 3tfc coloured by chain, side view.
PDB entry 3tfc coloured by chain, top view.
Source organism: Listeria monocytogenes EGD-e
Primary publication:
Structural analysis of a 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase with an N-terminal chorismate mutase-like regulatory domain.
Light SH, Halavaty AS, Minasov G, Shuvalova L, Anderson WF
Protein Sci 21 887-95 (2012)
PMID: 22505283

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186973 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chorismate mutase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 385 amino acids
Theoretical weight: 42.48 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8Y6T2 (Residues: 1-361; Coverage: 100%)
Gene name: aroA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand PEP 2 x PEP
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 110.161Å b: 110.89Å c: 80.23Å
α: 90° β: 127.21° γ: 90°
R-values:
R R work R free
0.176 0.175 0.205
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

The diversity of allosteric controls at the gateway to aromatic amino acid biosynthesis.
Light et al. (2013)
2 more

3 mentions without citation

Structural analysis of a 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase with an N-terminal chorismate mutase-like regulatory domain.
Light et al. (2012)
2 more