› 3tf0

X-ray diffraction
1.74Å resolution

Crystal structure of an H-NOX protein from T. tengcongensis

Released:
DOI: 10.2210/pdb3tf0/pdb
PDB entry 3tf0 coloured by chain, front view.
PDB entry 3tf0 coloured by chain, side view.
PDB entry 3tf0 coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Primary publication:
Tunnels modulate ligand flux in a heme nitric oxide/oxygen binding (H-NOX) domain.
Winter MB, Herzik MA, Kuriyan J, Marletta MA
Proc Natl Acad Sci U S A 108 E881-9 (2011)
PMID: 21997213

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting transducer domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 22.05 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RBX6 (Residues: 1-188; Coverage: 31%)
Gene names: TTE0680, Tar4
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments

3 bound ligands:
Ligand HEM 2 x HEM
Ligand OXY 2 x OXY
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21212
Unit cell:
a: 80.502Å b: 130.766Å c: 42.736Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.229
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Structure and regulation of soluble guanylate cyclase.
Derbyshire et al. (2012)
11 more

7 mentions without citation

An enzymatic method for precise oxygen affinity measurements over nanomolar-to-millimolar concentration regime.
Sanyal et al. (2020)
6 more