› 3tez

X-ray diffraction
1.83Å resolution

Crystal Structure of Anthrax Protective Antigen Mutant S337C N664C and dithiolacetone modified to 1.8-A resolution

Released:
DOI: 10.2210/pdb3tez/pdb
PDB entry 3tez coloured by chain, front view.
PDB entry 3tez coloured by chain, side view.
PDB entry 3tez coloured by chain, top view.
Source organism: Bacillus anthracis
Primary publication:
Domain flexibility modulates the heterogeneous assembly mechanism of anthrax toxin protective antigen.
Feld GK, Kintzer AF, Tang II, Thoren KL, Krantz BA
J Mol Biol 415 159-74 (2012)
PMID: 22063095

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand MXE 2 x MXE
Ligand ACN 1 x ACN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 71.293Å b: 93.686Å c: 117.851Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.226
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Ratcheting up protein translocation with anthrax toxin.
Feld et al. (2012)
4 more

3 mentions without citation

Domain flexibility modulates the heterogeneous assembly mechanism of anthrax toxin protective antigen.
Feld et al. (2012)
2 more