› 3tb4

X-ray diffraction
1.35Å resolution

Crystal structure of the ISC domain of VibB

Released:
DOI: 10.2210/pdb3tb4/pdb
PDB entry 3tb4 coloured by chain, front view.
PDB entry 3tb4 coloured by chain, side view.
PDB entry 3tb4 coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Structural insight into the ISC domain of VibB from Vibrio cholerae at atomic resolution: a snapshot just before the enzymatic reaction.
Liu S, Zhang C, Li N, Niu B, Liu M, Liu X, Wei T, Zhu D, Huang Y, Xu S, Gu L
Acta Crystallogr D Biol Crystallogr 68 1329-38 (2012)
PMID: 22993087

Function and Biology Details

Reaction catalysed: 
Isochorismate + H(2)O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vibriobactin-specific isochorismatase Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 24.84 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C6D3 (Residues: 1-215; Coverage: 73%)
Gene names: VC_0771, vibB
Sequence domains: Isochorismatase family
Structure domains: Isochorismatase-like

Ligands and Environments

5 bound ligands:
Ligand BOG 1 x BOG
Ligand PGE 1 x PGE
Ligand EDO 1 x EDO
Ligand PEG 1 x PEG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: P3221
Unit cell:
a: 55.657Å b: 55.657Å c: 118.746Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.136 0.134 0.166
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Unconventionally secreted effectors of two filamentous pathogens target plant salicylate biosynthesis.
Liu et al. (2014)
1 more

3 mentions without citation

An unconventionally secreted effector from the root knot nematode Meloidogyne incognita, Mi-ISC-1, promotes parasitism by disrupting salicylic acid biosynthesis in host plants.
Qin et al. (2022)
2 more