› 3t30

X-ray diffraction
1.9Å resolution

Human nucleoplasmin (Npm2): a histone chaperone in oocytes and early embryos

Released:
DOI: 10.2210/pdb3t30/pdb
PDB entry 3t30 coloured by chain, front view.
PDB entry 3t30 coloured by chain, side view.
PDB entry 3t30 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure and function of human nucleoplasmin (npm2): a histone chaperone in oocytes and embryos.
Platonova O, Akey IV, Head JF, Akey CW
Biochemistry 50 8078-89 (2011)
PMID: 21863821

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoplasmin-2 Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 110 amino acids
Theoretical weight: 12.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86SE8 (Residues: 14-122; Coverage: 51%)
Gene name: NPM2
Sequence domains: Nucleoplasmin/nucleophosmin domain
Structure domains: Nucleoplasmin core domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 49.03Å b: 107.24Å c: 108.7Å
α: 90° β: 102.54° γ: 90°
R-values:
R R work R free
0.193 0.192 0.243
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Nucleophosmin: from structure and function to disease development.
Box et al. (2016)
7 more

2 mentions without citation

Structure-function relationship of H2A-H2B specific plant histone chaperones.
Kumar et al. (2020)
1 more