› 3ss4

X-ray diffraction
2.85Å resolution

Crystal structure of mouse Glutaminase C, phosphate-bound form

Released:
DOI: 10.2210/pdb3ss4/pdb
PDB entry 3ss4 coloured by chain, front view.
PDB entry 3ss4 coloured by chain, side view.
PDB entry 3ss4 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Mitochondrial localization and structure-based phosphate activation mechanism of Glutaminase C with implications for cancer metabolism.
Cassago A, Ferreira AP, Ferreira IM, Fornezari C, Gomes ER, Greene KS, Pereira HM, Garratt RC, Dias SM, Ambrosio AL
Proc Natl Acad Sci U S A 109 1092-7 (2012)
PMID: 22228304

Function and Biology Details

Reaction catalysed: 
L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112596 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaminase kidney isoform, mitochondrial 65 kDa chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 479 amino acids
Theoretical weight: 53.33 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: D3Z7P3 (Residues: 128-602; Coverage: 70%)
Gene names: Gls, Gls1, Kiaa0838
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 97.72Å b: 136.28Å c: 176.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.27
Expression system: Escherichia coli

Quick links

Citations

56 review citations

Metabolic reprogramming: a cancer hallmark even warburg did not anticipate.
Ward et al. (2012)
55 more

3 mentions without citation

A tale of two glutaminases: homologous enzymes with distinct roles in tumorigenesis.
Katt et al. (2017)
2 more