› 3ss1

X-ray diffraction
2.2Å resolution

Clostridium difficile toxin A (TcdA) glucolsyltransferase domain

Released:
DOI: 10.2210/pdb3ss1/pdb
PDB entry 3ss1 coloured by chain, front view.
PDB entry 3ss1 coloured by chain, side view.
PDB entry 3ss1 coloured by chain, top view.
Source organism: Clostridioides difficile 630
Primary publication:
Structural determinants of Clostridium difficile toxin A glucosyltransferase activity.
Pruitt RN, Chumbler NM, Rutherford SA, Farrow MA, Friedman DB, Spiller B, Lacy DB
J Biol Chem 287 8013-20 (2012)
PMID: 22267739

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosyltransferase TcdA Chain: A
Molecule details ›
Chain: A
Length: 555 amino acids
Theoretical weight: 64.4 KDa
Source organism: Clostridioides difficile 630
Expression system: Priestia megaterium
UniProt:
  • Canonical: P16154 (Residues: 1-542; Coverage: 20%)
Gene names: tcdA, toxA
Sequence domains:
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

1 bound ligand:
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P65
Unit cell:
a: 141.908Å b: 141.908Å c: 65.946Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.227
Expression system: Priestia megaterium

Quick links

Citations

18 review citations

The Human Gut Microbiome - A Potential Controller of Wellness and Disease.
Kho et al. (2018)
17 more

9 mentions without citation

Clostridium difficile Toxins A and B: Insights into Pathogenic Properties and Extraintestinal Effects.
Di Bella et al. (2016)
8 more