› 3srd

X-ray diffraction
2.9Å resolution

Human M2 pyruvate kinase in complex with fructose 1-6 bisphosphate and Oxalate.

Released:
DOI: 10.2210/pdb3srd/pdb
PDB entry 3srd coloured by chain, front view.
PDB entry 3srd coloured by chain, side view.
PDB entry 3srd coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Morgan HP, O'Reilly F, Palmer R, McNae IW, Nowicki MW, Wear MA, Fothergill-Gilmore LA, Walkinshaw MD

Function and Biology Details

Reactions catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146985 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase PKM Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 551 amino acids
Theoretical weight: 60.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14618 (Residues: 1-531; Coverage: 100%)
Gene names: OIP3, PK2, PK3, PKM, PKM2
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand OXL 4 x OXL
Ligand FBP 4 x FBP
Ligand K 3 x K
Ligand MG 4 x MG
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 95.05Å b: 117.37Å c: 110.46Å
α: 90° β: 113.56° γ: 90°
R-values:
R R work R free
0.254 0.253 0.279
Expression system: Escherichia coli BL21(DE3)

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