› 3sph

X-ray diffraction
3Å resolution

Inward rectifier potassium channel Kir2.2 I223L mutant in complex with PIP2

Released:
DOI: 10.2210/pdb3sph/pdb
PDB entry 3sph coloured by chain, front view.
PDB entry 3sph coloured by chain, side view.
PDB entry 3sph coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structural basis of PIP2 activation of the classical inward rectifier K+ channel Kir2.2.
Hansen SB, Tao X, MacKinnon R
Nature 477 495-8 (2011)
PMID: 21874019

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123320 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-sensitive inward rectifier potassium channel 12 Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 39.36 KDa
Source organism: Gallus gallus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: F1NHE9 (Residues: 37-372; Coverage: 78%)
Gene name: KCNJ12
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand K 4 x K
Ligand PIO 1 x PIO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I4
Unit cell:
a: 82.725Å b: 82.725Å c: 185.192Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.254
Expression system: Komagataella pastoris

Quick links

Citations

96 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
95 more

9 mentions without citation

Structural basis of PIP2 activation of the classical inward rectifier K+ channel Kir2.2.
Hansen et al. (2011)
8 more