› 3sjn

X-ray diffraction
1.9Å resolution

Crystal structure of enolase Spea_3858 (target EFI-500646) from Shewanella pealeana with magnesium bound

Released:
DOI: 10.2210/pdb3sjn/pdb
PDB entry 3sjn coloured by chain, front view.
PDB entry 3sjn coloured by chain, side view.
PDB entry 3sjn coloured by chain, top view.
Source organism: Shewanella pealeana ATCC 700345
Entry authors: Patskovsky Y, Kim J, Toro R, Bhosle R, Hillerich B, Seidel RD, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Zencheck WD, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108648 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mandelate racemase/muconate lactonizing enzyme C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 41.21 KDa
Source organism: Shewanella pealeana ATCC 700345
Expression system: Escherichia coli
UniProt:
  • Canonical: A8H9D1 (Residues: 2-373; Coverage: 100%)
Gene name: Spea_3858
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand UNL 2 x UNL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I4
Unit cell:
a: 109.207Å b: 109.207Å c: 141.034Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.16 0.176
Expression system: Escherichia coli

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