3shv

X-ray diffraction
2.1Å resolution

Crystal structure of human MCPH1 tandem BRCT domains-gamma H2AX complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-147616 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Microcephalin Chains: A, B
Molecule details ›
Chains: A, B
Length: 206 amino acids
Theoretical weight: 22.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NEM0 (Residues: 639-835; Coverage: 24%)
Gene name: MCPH1
Structure domains: BRCT domain
Histone H2AX Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 amino acids
Theoretical weight: 1.24 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P16104 (Residues: 134-143; Coverage: 7%)
Gene names: H2AFX, H2AX

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 122.772Å b: 132.86Å c: 31.534Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.242
Expression systems:
  • Escherichia coli
  • Not provided