› 3sgc

X-ray diffraction
2.05Å resolution

Crystal Structure of Apo Aminoglycoside-2''-Phosphotransferase Type IVa

Released:
DOI: 10.2210/pdb3sgc/pdb
PDB entry 3sgc coloured by chain, front view.
PDB entry 3sgc coloured by chain, side view.
PDB entry 3sgc coloured by chain, top view.
Source organism: Enterococcus casseliflavus
Primary publication:
Crystal structures of antibiotic-bound complexes of aminoglycoside 2''-phosphotransferase IVa highlight the diversity in substrate binding modes among aminoglycoside kinases.
Shi K, Houston DR, Berghuis AM
Biochemistry 50 6237-44 (2011)
PMID: 21678960

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 35.68 KDa
Source organism: Enterococcus casseliflavus
Expression system: Escherichia coli
UniProt:
  • Canonical: O68183 (Residues: 1-296; Coverage: 98%)
Gene name: aph(2'')-Id
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 50.892Å b: 61.725Å c: 102.918Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.262
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Resilience of biochemical activity in protein domains in the face of structural divergence.
Zhang et al. (2014)
3 more

1 mention without citation

Strategies to overcome the action of aminoglycoside-modifying enzymes for treating resistant bacterial infections.
Labby et al. (2013)