› 3scg

X-ray diffraction
3Å resolution

Fe(II)-HppE with R-HPP

Released:
DOI: 10.2210/pdb3scg/pdb
PDB entry 3scg coloured by chain, front view.
PDB entry 3scg coloured by chain, side view.
PDB entry 3scg coloured by chain, top view.
Source organism: Streptomyces wedmorensis
Primary publication:
Structural basis of regiospecificity of a mononuclear iron enzyme in antibiotic fosfomycin biosynthesis.
Yun D, Dey M, Higgins LJ, Yan F, Liu HW, Drennan CL
J Am Chem Soc 133 11262-9 (2011)
PMID: 21682308

Function and Biology Details

Reaction catalysed: 
(S)-2-hydroxypropylphosphonate + H(2)O(2) = (1R,2S)-1,2-epoxypropylphosphonate + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(S)-2-hydroxypropylphosphonic acid epoxidase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 198 amino acids
Theoretical weight: 21.36 KDa
Source organism: Streptomyces wedmorensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56185 (Residues: 1-198; Coverage: 100%)
Gene names: fom4, hppE
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand TB6 3 x TB6
Ligand FE2 3 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1, null
Spacegroup: P42212
Unit cell:
a: 111.57Å b: 111.57Å c: 151.334Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.258
Expression system: Escherichia coli

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Citations

5 review citations

Enzymatic chemistry of cyclopropane, epoxide, and aziridine biosynthesis.
Thibodeaux et al. (2012)
4 more