› 3s5l

X-ray diffraction
2.1Å resolution

Crystal structure of CD4 mutant bound to HLA-DR1

Released:
DOI: 10.2210/pdb3s5l/pdb
PDB entry 3s5l coloured by chain, front view.
PDB entry 3s5l coloured by chain, side view.
PDB entry 3s5l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Affinity maturation of human CD4 by yeast surface display and crystal structure of a CD4-HLA-DR1 complex.
Wang XX, Li Y, Yin Y, Mo M, Wang Q, Gao W, Wang L, Mariuzza RA
Proc Natl Acad Sci U S A 108 15960-5 (2011)
PMID: 21900604

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-134522 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
HLA class II histocompatibility antigen, DR alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 182 amino acids
Theoretical weight: 21.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01903 (Residues: 26-207; Coverage: 80%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DRB1 beta chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 193 amino acids
Theoretical weight: 22.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01911 (Residues: 30-221; Coverage: 81%)
Gene name: HLA-DRB1
Sequence domains:
Structure domains:
Hemagglutinin HA1 chain Chains: C, F
Molecule details ›
Chains: C, F
Length: 13 amino acids
Theoretical weight: 1.51 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P03437 (Residues: 322-334; Coverage: 2%)
Gene name: HA
T-cell surface glycoprotein CD4 Chains: G, H
Molecule details ›
Chains: G, H
Length: 191 amino acids
Theoretical weight: 21.37 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P01730 (Residues: 26-203; Coverage: 41%)
Gene name: CD4
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand GOL 10 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 94.2Å b: 119.7Å c: 137.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.268
Expression systems:
  • Escherichia coli
  • Not provided
  • Spodoptera frugiperda

Quick links

Citations

10 review citations

The structural basis of αβ T-lineage immune recognition: TCR docking topologies, mechanotransduction, and co-receptor function.
Wang et al. (2012)
9 more

5 mentions without citation

Strict Major Histocompatibility Complex Molecule Class-Specific Binding by Co-Receptors Enforces MHC-Restricted αβ TCR Recognition during T Lineage Subset Commitment.
Li et al. (2013)
4 more