› 3rym

X-ray diffraction
1.7Å resolution

Structure of Oxidized M98K mutant of Amicyanin

Released:
DOI: 10.2210/pdb3rym/pdb
PDB entry 3rym coloured by chain, front view.
PDB entry 3rym coloured by chain, side view.
PDB entry 3rym coloured by chain, top view.
Source organism: Paracoccus denitrificans
Primary publication:
Replacement of the axial copper ligand methionine with lysine in amicyanin converts it to a zinc-binding protein that no longer binds copper.
Sukumar N, Choi M, Davidson VL
J Inorg Biochem 105 1638-44 (2011)
PMID: 22071089

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149611 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amicyanin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 105 amino acids
Theoretical weight: 11.5 KDa
Source organism: Paracoccus denitrificans
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P22364 (Residues: 27-131; Coverage: 100%)
Gene names: ami, mauC
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 37.367Å b: 51.668Å c: 56.543Å
α: 105.97° β: 96.48° γ: 108.35°
R-values:
R R work R free
0.192 0.19 0.215
Expression system: Escherichia coli BL21

Quick links

Citations

2 citation in other articles

Zinc-mediated allosteric inhibition of caspase-6.
Velázquez-Delgado et al. (2012)
1 more

2 mentions without citation

Replacement of the axial copper ligand methionine with lysine in amicyanin converts it to a zinc-binding protein that no longer binds copper.
Sukumar et al. (2011)
1 more