› 3ry1

X-ray diffraction
1.03Å resolution

Wild-type core streptavidin at atomic resolution

Released:
DOI: 10.2210/pdb3ry1/pdb
PDB entry 3ry1 coloured by chain, front view.
PDB entry 3ry1 coloured by chain, side view.
PDB entry 3ry1 coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Streptavidin and its biotin complex at atomic resolution.
Le Trong I, Wang Z, Hyre DE, Lybrand TP, Stayton PS, Stenkamp RE
Acta Crystallogr D Biol Crystallogr 67 813-21 (2011)
PMID: 21904034

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 127 amino acids
Theoretical weight: 13.28 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 37-163; Coverage: 80%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

2 bound ligands:
Ligand MPD 3 x MPD
Ligand MRD 7 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 58.018Å b: 84.432Å c: 45.994Å
α: 90° β: 98.81° γ: 90°
R-values:
R R work R free
0.118 0.117 0.135
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Atomistic Simulations of Functionalized Nano-Materials for Biosensors Applications.
Dutta et al. (2022)
2 more

15 mentions without citation

Plug-and-play pairing via defined divalent streptavidins.
Fairhead et al. (2014)
14 more